《植物生理学报》 2009, 45(6): 567-570

外源一氧化碳对小麦幼苗耐盐性以及根中脯氨酸含量的影响

袁星星, 王娟, 谢彦杰, 沈文飚*

南京农业大学生命科学学院, 南京210095

通信作者：沈文飚；E-mail: wbshenh@njau.edu.cn；Tel: 025-84396542

摘　要：

小麦幼苗经饱和度为 50% 的一氧化碳(CO)溶液预处理 24 h 可以缓解随后以 200 mmol•L-1 NaCl处理所导致的小麦幼 苗生长的受抑程度和相对含水量的下降。CO 预处理还可有效提高盐胁迫下小麦幼苗根中吡咯啉-5-羧酸合成酶(P5CS)活 性及其基因的表达, 同时抑制脯氨酸脱氢酶(ProDH)活性, 从而诱导脯氨酸的大量合成, 缓解盐胁迫对小麦幼苗的伤害

关键词：盐胁迫; 小麦; 一氧化碳; 脯氨酸

收稿：2009-03-13   修定：2009-04-23

资助：国家自然科学基金(30671248)。

Effects of Carbon Monoxide on Salt Tolerance and Proline Content of Roots in Wheat Seedling

YUAN Xing-Xing, WANG Juan, XIE Yan-Jie, SHEN Wen-Biao*

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: SHEN Wen-Biao; E-mail: wbshenh@njau.edu.cn; Tel: 025-84396542

Abstract:

In present study, we discovered that wheat seedlings subjected to 50% CO aqueous solution pretreatment for 24 h, led to a significant reversal in dry weight and water loss caused by 200 mmol·L^-1 NaCl treatment. Further analysis showed that CO pretreatment apparently up-regulated Δ¹-pyrroline-5-carboxylate synthetase (P5CS) activity and its related transcripts, and inhibited proline dehydrogenase (ProDH) activity, thus resulting in the increase of proline content and the alleviation of salinity-induced damage in the root tissues.

Key words: salt stress; wheat (Triticum aestivum); carbon monoxide; proline